Cysteine lactose electrolyte deficient agar
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(Redirected from CLED agar)
CLED agar (cysteine lactose electrolyte deficient medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary organisms. Being electrolyte deficient, it prevents the swarming of Proteus species. Cysteine promotes the formation of cysteine-dependent dwarf colonies. Lactose fermenters produce yellow colonies on CLED agar; non-lactose fermenters appear blue. It has a pH of approximately 7.3. CLED agar contains:
Peptone
4g/l
'Lab Lemco' powder
3g/l
Tryptone
4g/l
Lactose
10g/l
L-Cysteine
128mg/l
Bromothymol blue
20mg/l
Agar No. 1
15g/l
v?d?eGrowth media / agar plates
Selective media
MacConkey agar - Buffered charcoal yeast extract agar - Thayer-Martin agar - Bile esculin agar - Lowenstein-Jensen medium - XLD agar - Cetrimide agar - Hoyle's Agar
Differential media
Eosin methylene blue - MacConkey agar/Sorbitol-MacConkey agar - Bile esculin agar - Hektoen enteric agar
Fungal
Sabouraud agar - Potato dextrose agar
Other/ungrouped
Cysteine lactose electrolyte deficient agar - R2a agar - Trypticase soy agar - Nutrient agar - Plate count agar - Chocolate agar - Eaton's agar - Bismuth sulfite agar - Simmons' citrate agar - DCA agar - XLT agar - XLD agar - PNP agar - VRBDA - Dermatophyte Test Medium - Bordet-Gengou agar - Mueller-Hinton agar - Cystine tryptic agar
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